紅麴保護循環骨髓源性血管生成細胞免受高葡萄糖誘導的衰老和氧化壓力：血鐵質氧化酶-1的作用
Oxidative Medicine and Cellular Longevity, 2017; 2017: 3831750.
The inflammation and oxidative stress of bone marrow-derived proangiogenic cells (PACs), also named endothelial progenitor cells, triggered by hyperglycemia contributes significantly to vascular dysfunction. There is supporting evidence that the consumption of red yeast rice (RYR; Monascus purpureus-fermented rice) reduces the vascular complications of diabetes; however, the underlying mechanism remains unclear. This study aimed to elucidate the effects of RYR extract in PACs, focusing particularly on the role of a potent antioxidative enzyme, heme oxygenase-1 (HO-1). We found that treatment with RYR extract induced nuclear factor erythroid-2-related factor nuclear translocation and HO-1 mRNA and protein levels in PACs. RYR extract inhibited high-glucose-induced PAC senescence and the development of reactive oxygen species in a dose-dependent manner. The HO-1 inducer cobalt protoporphyrin IX also decreased high-glucose-induced cell senescence and oxidative stress, whereas the HO-1 enzyme inhibitor zinc protoporphyrin IX and HO-1 small interfering RNA significantly reversed RYR extract-caused inhibition of senescence and reduction of oxidative stress in high-glucose-treated PACs. These results suggest that RYR extract serves as alternative and complementary medicine in the treatment of these diseases, by inducing HO-1, thereby decreasing the vascular complications of diabetes.
Oxidative Medicine and Cellular Longevity, 2017; 2017: 3831750.
由高血糖引發的內皮先驅細胞（也稱作骨髓源性促血管生成細胞）的發炎和氧化壓力會顯著導致血管功能障礙。有研究證據顯示紅麴（Monascus purpureus發酵米）可減少糖尿病相關的血管併發症，然而潛在的機制仍不清楚。本研究旨於闡明紅麴萃取物在內皮先驅細胞的作用，特別關注有細胞保護潛力之抗氧化酵素血鐵質氧化酶-1（heme oxygenase-1; HO-1）的機轉。我們發現用紅麴萃取物處理誘導轉錄核因子erythroid-2相關因子之核易位以及內皮先驅細胞中的血鐵質氧化酶-1 mRNA和蛋白質表現。紅麴萃取物並以劑量依賴性方式抑制高葡萄糖誘導的內皮先驅細胞衰老和活性氧的產生。血鐵質氧化酶-1之誘導劑（鈷原卟啉IX）還降低了高葡萄糖糖所誘導的細胞衰老和氧化壓力；而血鐵質氧化酶-1之酶抑製劑（鋅原卟啉 IX）和血鐵質氧化酶-1短干擾核糖核酸顯著逆轉了紅麴萃取物引起的衰老抑制，和減少高葡萄糖誘導之內皮先驅細胞氧化壓力。這些結果表明紅麴萃取物有潛力作為治療這些疾病的替代和補充藥物，通過誘導血鐵質氧化酶-1表現，進而減少糖尿病相關的血管併發症。